http://triscience.com In vivo is a Latin phrase that literally translates to “within the living.” It is a type of experiment that uses the whole living organism instead of just a part of a body or a cadaver. In vivo is the direct opposite of an in vitro procedure (Latin for “within the glass,” more commonly used to refer to experiments done in a petri dish or a test tube). Two of the most common forms of in vivo research are clinical trials and animal testing. Many scientists prefer in vivo testing over in vitro procedures because the overall effect of a specific experiment is easily observable on a living subject. In the field of microbiology, in vivo is more commonly used to refer to the experiments performed on live isolated cells instead of the whole organism. An in vivo procedure typically uses cultured cells culled from biopsies. However, this practice is more specifically known as an ex vivo (Latin for “out of the living) procedure. Ex vivo is an experiment or measurement done “in or on a tissue in an artificial environment outside the organism with the minimum alteration of natural conditions.” Scientists can experiment under more controlled conditions compared to in vivo experiments—however, the natural environment can be altered. Ex vivo experiments are more preferable than in vivo ones because scientists will be able to perform measurements and tests that will not be ethically possible in living subjects. Tissues for an ex vivo test can be removed through a wide variety of ways, including by part, by removing the whole organs, or removing larger organ systems. Ex vivo specimen uses include assays, realistic models for the development of surgical procedures, investigating the interactions of different energy types with an organism’s tissues, and measuring tissue properties in different kinds of extreme environments. This category contains scientific information about the processes of in vivo and ex vivo, which are experimentation procedures that use organisms which are organizations of living cells. daily 1 2012-06-18T07:39:14Z http://triscience.com/Cells/Vivo/sequential-immunizations-with-rgp120s-from-independent-isolates-of-human-immunodeficiency-virus-type-1-induce-the-preferential-expansion-of-broadly-crossreactive-b-cells The gp120 envelope glycoprotein of human immunodeficiency virus type 1 (Hiv-1) is a dominant target against which the host's humoral immune response is directed. Unfortunately, gp120 proteins from dif... No publisher admin activation antibodies antigenically balbc cell cells crossreacted crossreactive dominant elisa enzyme glycoprotein human humoral iiib immune immunization immunizations immunized immunizing 2013-05-19T04:14:41Z Doculite http://triscience.com/Cells/Vivo/separation-of-periportal-and-perivenous-rat-hepatocytes-by-fluorescence-activated-cell-sorting-confirmation-with-colloidal-gold-as-an-exogenous-marker Periportal and perivenous hepatocytes are known to display various functional differences. In this study we present a new method to separate periportal and perivenous cells: after selectively loading ... No publisher admin acinar acridine antegrade biochemically cell cells collagenase colloidal competing enzyme enzymes fluorescence fluorescent fractions gold granules hepatocytes histochemically histochemistry kupffer 2013-05-19T04:14:15Z Doculite http://triscience.com/Cells/Vivo/separation-of-limonene-and-selinene-cyclases-by-ion-exchange-chromatography The enzymes limonene cyclase and.beta.-selinene cyclase are present in the supernatant extracted from the epicarp of Calamondin (Citrofortunella mitis) fruits. The two enzymes were extracted and purif... No publisher admin 20 calamondin catalyzed chromatography citrofortunella cyclase cyclization enzymes epicarp extracted farnesyl fruits geranyl limonene mitis purified pyrophosphate selinene 2013-05-19T04:14:13Z Doculite http://triscience.com/Cells/Vivo/separation-of-human-serum-lipoproteins-into-three-major-classes-by-hydroxyapatite-chromatography The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6-7.4 wer... No publisher admin 20 density eluents fraction hydroxyapatite lipoprotein lipoproteins normolipidemic phosphate potassium serum tiselius ultracentrifugal 2013-05-19T04:14:12Z Doculite http://triscience.com/Cells/Vivo/separation-of-epimeric-pairs-of-c-24-alkylsterols-by-reversed-phase-high-performance-liquid-chromatography-of-the-free-sterols-at-subambient-temperature Separation of most of the common biologically occurring epimeric pairs of C-24 alkylsterols was obtained by reversed-phase Hplc of non-derivatized free sterols at subambient temperatures. Base line se... No publisher admin 20 alkylsterols biologically cholesterol dehydrocholesterol derivatized desmosterol epimeric ethylsterols hplc isopropanol lathosterol metabolic metabolism methanol solvent sterols subambient temperatures 2013-05-19T04:14:11Z Doculite http://triscience.com/Cells/Vivo/separation-and-identification-of-various-vulcanization-agents-and-antioxidants-in-two-types-of-rubber-by-chromatographic-and-spectrometric-methods The aim of this study was to separate and identify by chromatographic and spectrometric methods, the various allergenic vulcanization agents and antioxidants used in the manufacture of industrial rubb... No publisher admin 20 allergenic chromatographic elastomers products specimens spectrometric spectrometry vulcanization 2013-05-19T04:14:07Z Doculite http://triscience.com/Cells/Vivo/separated-flow-demonstrated-by-digitized-cineangiography-compared-with-ldv In order to demonstrate separated flow in vivo, a method for the computerized analysis of cineangiographies has been developed, tested in vitro, and compared with Ld A pulsatile flow was created in a ... No publisher admin 20 cineangiographies cineangiography cinefilmed pulsatile sequence vitro vivo 2013-05-19T04:14:05Z Doculite http://triscience.com/Cells/Vivo/sensitivity-of-actinobacillus-actinomycetemcomitans-and-haemophilus-aphrophilus-to-oxidative-killing We examined the killing of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by oxygen metabolites generated by the xanthine-xanthine oxidase (X-Xo) system. This system generates a mixt... No publisher admin 20 actinomycetemcomitans aphrophilus bactericidal catalase cytochrome desferrioxamine dimethyl dismutase hydrogen hydroxyl lessers oxidant oxidants oxidase oxygen peroxide produced production serotypes sulfoxide 2013-05-19T04:13:49Z Doculite http://triscience.com/Cells/Vivo/senescence-behavior-of-some-rice-cultivars Short-statured rice (Oryza sativa L.) cultivars Rasi (Iet 1444) and Sashyasri showed a nonsequential mode of leaf senescence behaviour, where the total leaf area, number of vascular bundles at the jun... No publisher admin 20 badsabhog behaviour cultivars kalma kalojira lamina leaf lumen nonsequential oryza patnai rasi sashyasri sativa senesced senescence statured vascular xylem 2013-05-19T04:13:43Z Doculite http://triscience.com/Cells/Vivo/selenocysteine-synthase-from-escherichia-coli-nucleotide-sequence-of-the-gene-sel-a-and-purification-of-the-protein The nucleotide sequence of the selA gene from Escherichia coli whose product is involved in the conversion of seryl-tRNAUCASec into selenocysteyl-tRNAUCASec was determined. selA codes for a polypeptid... No publisher admin acid cells coli escherichia gene migrates nucleotide phage phosphatemol plasmid polypeptide product promoter promoterpolymerase protein purification purified pyridoxal sela selenium 2013-05-19T04:13:28Z Doculite http://triscience.com/Cells/Vivo/selective-up-regulation-of-macrophage-function-in-granulocyte-macrophage-colony-stimulating-factor-transgenic-mice Peritoneal and pleural cells from mice transgenic for Gm-Csf were studied with regard to their phenotype and functional capacity, and compared with cells from normal littermates. Transgenic mice showe... No publisher admin activation cell cells constrast cytometry cytoplasm cytotoxicity drug expression littermate littermates macrophage macrophages mice mouse pathology peritoneal phenotype populations production 2013-05-19T04:13:21Z Doculite http://triscience.com/Cells/Vivo/selective-inhibition-of-cysteine-proteinases-by-z-phe-alach-2f-suppresses-digestion-of-collagen-by-fibroblasts-and-osteoclasts Effects of the selective inhibitor of cathespins B and L, Z-Phe-AlaCH2F were studied on the degradation of fibrillar collagen by fibroblasts and osteoclasts in cultured rabbit calvariae at the electro... No publisher admin calvariae cathespins cells concentration cytotoxic degradation electron explants fibrillar fibrils fibroblasts fraction inhibitor intralysosomally microscopic osteoclasts periosteal protein rabbit vacuoles 2013-05-19T04:13:12Z Doculite http://triscience.com/Cells/Vivo/selective-fascicular-nerve-repair-a-rapid-method-for-intraoperative-motor-sensory-differentiation-by-acetylcholinesterase-histochemistry The selective reunion of motor and sensory fascicles of severed mixed nerves appears indispensible for optimal recovery of the impaired motor function. Procedures available for rapid identification of... No publisher admin 20 acetylcholinesterase clinical concentration fascicles fascicular histochemical histochemistry incubation indispensible intraoperative motorsensory temperature 2013-05-19T04:13:08Z Doculite http://triscience.com/Cells/Vivo/selective-depletion-of-macrophages-prevents-pituitary-adrenal-activation-in-response-to-subpyrogenic-but-not-to-pyrogenic-doses-of-bacterial-endotoxin-in-rats The mechanisms by which bacterial endotoxin stimulates the hypothalamo-pituitary-adrenal axis (Hpaa) have not been elucidated. The present study was designed to investigate the involvement of macropha... No publisher admin acid acth activation animals antibodies bacterial catecholamine cells concentration concentrations corticosterone corticosteronekg cytospins dichloromethylene diphosphonate encapsulated endotoxin epinephrine exudates granulocytes 2013-05-19T04:13:04Z Doculite http://triscience.com/Cells/Vivo/selective-cytotoxicity-of-n-acetyl-4-s-cysteaminylphenol-on-follicular-melanocytes-of-black-mice Previous in vivo studies have shown that 4-S-cysteaminylphenol (4-S-Cap) and N-acetyl-4-S-cysteaminylphenol (N-Ac-4-S-Cap) have antimelanoma effects and that N-Ac-4-S-Cap produced a 98% depigmentation... No publisher admin acetyl antimelanoma cells cysteaminylphenol cytoplasm cytotoxic depigmentation detruction drug electron eumelanin granules intraperitoneal keratonocytes light melanocytes melanocytic melanocytotoxicity membranous mice 2013-05-19T04:13:03Z Doculite